Category: BioTechnology

Making Bone With An Ink Jet Printer. Well…Bone-ish.

Reading the headline, “Engineers Pioneer Use of 3D Printer To Create New Bones” from the BBC I can’t help but imagine your standard ink jet spitting out layers of human bone until you come up with a whole femur. In case you aren’t familiar with 3D cell printing, let me be the one to tell you that isn’t the case. I think the BBC‘s headline leaves out a crucial piece of information: what the printer in question creates is a scaffold of bone-like material.

The research in the article was conducted at Washington State University, and I find their PR headline “3D Printer Used To Make Bone-like Material” more specific. I think 3D printing, tinkering with a printer so that it can make different kinds of biomaterials, is interesting in its own right. I’m okay with the fact that the material being made is only bone-ish and not really bone. Although and argument could be made for the BBC’s headline… which I’ll explain later.

Printing the bone scaffold via WSU

Here is the research rundown: led by Susmita Bose, professor of mechanical and materials engineering, WSU researchers used a 3D printer to to create a scaffold of calcium phosphate, silicon and zinc. When paired with actual bone, this scaffold provides a structure for new bone to grow on, to specifically manufacture the desired bone. The scaffold dissolves with no reported adverse effects, according to the researchers’ in vitro tests in rats and rabbits.

Described in the journal Dental Materials, (according to the PR*) the printer works by having the inkjet spray a plastic binder over a layer of the calcium phosphate, silicon and zinc powder in very thin layers (about 20 microns, comparable to the width of a human hair). A computer directs the printer to create the scaffold in the desired shape and size. The researchers found that after a week in a medium containing immature human bone cells, the scaffold was able to support new bone cell growth. According to the researchers, the material is likely most suitable for low load bearing (so, not a femur) and could be available for human use in a few years time.

So back to the BBC’s headline about the 3D printer creating new bone. Ultimately, that is what happens. New bone is grown around the scaffold, so the end product is real human bone. However, the printer is not itself printing bone. In my humble opinion, that doesn’t make this research any less cool. While the BBC‘s headline wasn’t itself inaccurate, I think it leaves a lot of wiggle room for assumptions (or at least imaginations like mine getting carried away with themselves) and accuracy is the end all and be all of science stories, isn’t it? Something like “3D Printer Creates Scaffold For New Bone Growth” isn’t as pretty as either headline used, but I think it would get to the heart of what this story is a little bit better.

For more information about the technology check out this video from WSU’s press page:
*I am typically loathe to post about a paper that I haven’t at least looked at the abstract, but I cannot find this paper online anywhere. If someone has a link, that would be awesome. 

BioTechnology Patents: Kyoto Claims iPSCs

Can you patent a gene? What about a cell? When it comes to the components of life, and more importantly the ideas, processes, and procedures developed to manipulate these components, what belongs to who? This is a question that is certainly going to be fought out from the patent office to the courts as more and more biomedical discoveries are made.

iPS cell cluster. Source: NINDS.NIH.gov

One discovery that has recently (meaning August) been in the news for patent applications is Shinya Yamanaka’s 2006 discovery of the combination of genes that can be used to reprogram adult cells to a pluripotent (capable of becoming any kind of cell) state. With these induced pluripotent stem cells (iPSCs) lies the hope of a suitable answer to the debate over the need for pluripotency, but the social and religious controversy over using human embryonic stem cells (which are naturally pluripotent).

While working at Kyoto University in Japan, Yamanaka found that the genes Oct3/4, SOX2, c-Myc, and Klf4 are key to pluripotency. This discovery led to the creation of the first iPSCs. Now, five years and millions of dollars in research later, Kyoto University has obtained patent rights for iPSC technology in six nations and two regions, including the United States. This development leads me back to the question I started with, can you patent a gene? What about the ideas or technology based on those genes? Apparently, you can because Kyoto University has. But, I’m still quite curious about how this will play out functionally.

The discovery of iPSCs was huge news. It prompted researchers around the world to start working with iPSCs, many of whom have subsequently made their own discoveries, published their own research in peer reviewed journals (just type pluripotent into PubMed you’ll see what I mean), and expanded greatly on the existing body of knowledge about pluripotency. This includes the discovery of numerous variations of gene combinations that play a role in pluripotency. So if Kyoto University owns the original idea, do they own everyone elses’ work too? According to university spokeswoman Akemi Nakamura, they do. Nakamura says the patent broadly covers variations of the technology developed since 2006 in laboratories around the world.

In a press release the University stated:

“The US patent covers combinations of nuclear reprogramming family factors comprising an Oct family gene, a Klf family gene, and Myc family gene; or an Oct family gene, a Klf family gene, and a cytokine. This means that if companies use a combination of the nuclear reprogramming genes and generate iPSCs, regardless of the kinds of vectors, they need to get the patent license.”

So if Kyoto University owns the right to the genes, and the subsequent developments based on the genes what does that mean for iPSC researchers? Right now the university says it will not restrict research using iPSCs for non-profit purposes, so that would mean research whose end goal isn’t the marketing of a specific product based on iPSC technology will be able to continue unhindered. Companies that want to work with iPSCs for profit may have to pay a licensing fee. Although, it is important to note that not all iPSC research is based on these genes – there are other combinations of genes that can induce pluripotency, and thus lines of inquiry in this field that don’t belong to Kyoto University.

How important all of this will be, and when it will be important is a bit murky. iPSCs have their own problems (namely, teratomas) and haven’t yet been developed for widespread, let alone commercial, use. Though, with all of the resources being poured into iPSC development, I think it is only a matter of time until the cells become more useable. This is a story to watch, it is hard to say exactly how it will work out but it is sure to be an issue that continues to come up.

As for me, I’m not really sure where I fall on this issue. I can see the need to protect intellectual achievements and make sure that the wrong people don’t profit, but at the same time I wish it wasn’t necessary and open inquiries could be pursued without people having to worry about others cashing in on their ideas. If only it could be that way.

A Pollution Solution, Brought To You By Lehigh University

The Lehigh Mountain Hawk in 2008
photo credit: Erin Podolak

If you’ve ever checked the About section of this blog, you’ll know that my alma mater is Lehigh University. I loved my time at Lehigh (it’s where I first learned about science writing) and thinking about the university evokes a lot of positive memories. But, as much as I love Lehigh, I have to admit it isn’t exactly a premier research institution (despite what they might tell you in the pamphlets). Not that research doesn’t go on at Lehigh, but it’s no University of Wisconsin-Madison as far as a reputation for cutting edge research is concerned.

Imagine my surprise as I was perusing Scientific American a few weeks ago when I stumbled upon Lehigh while reading an article (reprinted from ClimateWire) about a newly developed material that has the ability to pull carbon dioxide and methane pollution from other gases. The material was developed by Kai Landskron, Paritosh Mohanty and Lillian D. Kull of Lehigh’s department of chemistry, and could potentially be used to help capture greenhouse gases.

Creating carbon-sucking materials has been a goal for scientists for years as a way to combat the effects of climate change caused by an excess of greenhouse gases in the atmosphere. However, existing systems tend to be expensive, use a tremendous amount of energy, or don’t work well at high temperatures. The new material developed at Lehigh avoids these problems.

The new substance was created using chemicals called diaminobenzidine and hexachlorocyclotriphosphazene. These chemicals are cheaper than others used for carbon absorption, and can operate at heat as high as 400 degrees Celsius. In addition to avoiding the problems that have plagued early carbon capture systems the researchers also had to create something that could take carbon dioxide and methane out of a gas stream, but then release it at a later time for permanent storage underground once compressed.

Coal power plant Somerset NY
Credit: Matthew D. Wilson/Wikimedia Commons.

When they developed their “sponge” the researchers found that the material drew more carbon dioxide and methane from the air than other gases, like nitrogen. This makes the material idea for capturing harmful greenhouse gases out of mixed emissions. The researchers have suggested that the material could be placed inside a tower located adjacent to a coal burning power plant, the flue gas generated from the burning coal could then be transported via pipeline through the material to capture greenhouse gases from the emissions.

According to the researchers, the material has a 90% success rate capturing CO2 from a gas stream. However, some problems with the mass production of this material include the fact that real power plants would emit a more complex mixture of gases than was tested by the Lehigh research team, the material may be too dense for manufacture on a large enough scale, and production would create chemical byproducts that may become difficult to control.

The researchers are confident however, in the product they have created. Landskron told ClimateWire:
“There is no fundamental difference in doing this in the lab versus doing it at an industrial scale.” This material hasn’t been tested on a commercial scale and it remains unknown if it could actually be implemented practically, so we’ll have to wait and see if the material can stand up to the high expectations its creators have set up for it.

Even though the chemicals used in the material are cheaper than others used for carbon capture, the cost of producing and implementing the technology is still a barrier to its use. The researchers hoped to test the material on an existing coal plant in the US earlier this year, but the effort stalled due to a lack of funds, even with a 50% investment by the Department of Energy.

On campus with friends before my graduation from
Lehigh in 2009.

So, while the research is promising and it demonstrates an interesting idea with a lot of potential for carbon capture it needs support and further research to make it something that could actually be used commercially. If you’d like to know more, the research was published in July in Nature Communications.

I was excited to see Lehigh in the news for scientific research. Research wasn’t a big part of my life at Lehigh, in fact I rarely encountered it, but Lehigh is where my passion for science evolved into a career. It is where, with the support of the journalism department and the wonderful professors who gave me my first real introduction to writing, I realized that I could have a career dedicated to science without being a scientist, and that has shaped the course of my life. I’m proud of my school, and even prouder to know that Lehigh researchers are working to find solutions to our greenhouse gas problems. Now lets get some funding to make that research a reality!

The Robot That Walks On Water

I don’t talk about religion on Science Decoded (with one exception) the way that I don’t talk about politics (with one exception). So all Jesus walking on water references will be excluded from this post. Sorry if that disappoints. But, I am going to talk about a robot that walks on water, and that alone is pretty cool.

Credit: The American Chemical Society.

Researchers from the State Key Laboratory of Robotics and System, and the Harbin Institute of Technology in China, writing in the journal ACS Applied Materials & Interfaces, have developed a microbot that is able to walk across water’s surface. The robot was designed to mimic the capabilities of water-striding insects like mosquitoes that can support themselves on water’s delicate surface.

The Chinese microbot is approximately six inches long and has 10 wire legs and 2 moveable oar-like legs. It is propelled by two small motors that help it to maneuver like a water striding insect. What makes the robot so much more impressive than what the insects do is that at 3.88 grams it weighs about as much as 390 water strider bugs. Despite its weight it is still able to walk, stand and turn on water’s surface without sinking.

So what is the trick to walking on water? My favorite: Math. (Sarcasm intended). While I still might be a bit intimidated by math, I definitely appreciate the amazing ways that nature is really just math and vice versa. The microbot’s legs are able to support it the way a water strider’s legs can support it based on the radius and contact angle of the legs with the water’s surface.

But the real question here is: aside from the fact that a robot that walks on water is just cool, why does it matter? According to the researchers this type of technology could be useful for developing tools for monitoring water pollution or water quality surveillance. Personally, I’m envisioning little robot spies stealthily sneaking across bodies of water, but that is something the researchers didn’t speculate on.

It amazes me everyday the kind of advances we’ve made in robotics, as we automate the world around us I can’t help but borrow the tagline from my friend Cassi’s blog: We Live In The Future.

Prosthetic Devices: The Mystery of Human Design

In the course of an average day I go up and down the stairs in my apartment building, I walk to class, and I run errands – all on foot. Not having a car, or even a bike drives home just how much I rely on my legs to get me where I need to go. But what would I do, if simply getting up in the morning and walking to my destination wasn’t possible?

For millions of people in the world, it isn’t. When people say that we should appreciate our health, I think of viruses, cancer, heart disease, or mental illness – I rarely think of the fact that I have all of the parts of my body that I’m supposed to have. For those individuals who don’t have their legs either by a birth defect, traumatic accident or as a result of war, the loss of mobility changes everything about how you would go through your day.



via Wikimedia Commons

The leg, knee, ankle, and foot (the lower extremity) perform two biological functions – stability and mobility. The lower extremity is designed to hold the body’s weight. According to Dr. Mark Geil, Director of the biomechanics laboratory in the department of kinesiology and health at Georgia State University, the lower extremity is amazing in its ability to support the body given our height and the relatively small surface area provided by the foot. In addition to stability, the muscles in the leg are key to making us mobile at various speeds, and over a variety of terrains and conditions.

For people who don’t have or have lost their leg, replicating the stability and mobility of the natural leg is the challenge presented to the researchers who design prosthetic devices. Prosthetics are artificial legs made of metal and plastic that take the place of the lost limb. But what mechanical challenges does replicating the human body entail?

The process of creating an artificial limb that acts like a human leg is called biomimetic design. According to Dr. Geil, just the act of walking can be incredibly complex, so researchers study it to inform their designs. In some cases, the goal is not to get the artificial leg as anatomically similar to a real leg as possible – it is to get the functionality as similar as possible.

Dr. Geil gives the example of a foot, which uses controlled motion through eccentric action of a muscle, called the tibialis anterior, to absorb shock every time we take a step. A prosthetic foot/ankle unit has no muscles, so the function of shock absorption has to be replicated through materials built into the artificial foot that essentially has a solid, immobile ankle.

But are there elements of human design that cannot be replicated by the ingenuity, creativity, and dedication of researchers? The answer is quite simply, yes – muscles.

“Muscles are the only tissue that can actively produce force,” says Geil. “They are not just springs that can store energy and return it, they actively produce energy. There are a few powered prosthetic components available now, and they generate force, but they must do so via a heavy motor and batteries. Nothing comes close to the elegance and efficiency of muscle action.”

via US Army Flickr

According to Dr. Geil, replicating human anatomy and function is the “Holy Grail” for biomimetic design. But finding a way around the problem posed by muscles is only part of the problem. The human leg is designed to accomplish a vast array of activities – and this diversity has proved difficult to make possible with a single prosthetic leg.

“We think nothing of ascending and descending stairs, walking slowly or quickly and sometimes running, stopping, turning, sitting and standing. We walk on varied surfaces and up and down ramps. We climb ladders or kneel,” says Geil. “I believe that something we’re still missing, and something that might be designed into future prosthetics, is the adaptability required for different conditions and tasks.”

While the technology needed to give people who have lost a limb a prosthetic that can do all the things that a natural leg would be able to do isn’t available yet, there is progress being made to understand the way the human body works. The more researchers know about the body – the better they will be at making machines the act like the human body.

For all of human history we have been studying ourselves, trying to figure out how these bodies work – and there is still so much to know. The next time I take the stairs in my apartment building, and walk the few blocks to campus, I know I’ll have a new appreciation for the legs that are getting me there. Not just because I have them, when so many others don’t, but because I appreciate how beautifully, and still mysteriously, they are designed.